
The E5 Protocol
The E5 Protocol was designed by Jonny Bradley as a personal framework to optimise energy, longevity, and overall well-being.
Built on five essential pillars; Energy, Essentials, Enhancement, Evolution, and Equilibrium. The protocol offers a comprehensive approach to health and performance, helping you thrive at the cellular level.
Each pillar represents a vital aspect of Jonny’s health journey, working together to support cellular repair, energy production, and long-term resilience.
Discover how these elements combine to create lasting vitality and balance.
Jonny’s BioMarkers
Testing: A True Measure of Progress
Testing is critical because how you feel may not always accurately reflect your internal health. By tracking measurable data, we can get a true picture of how things are functioning at a cellular level.
Age Metrics
- Chronological Age: 34.8
- Biological Age: Waiting Latest Results
- Rate of Ageing: Waiting Latest Results
Cellular Health
- Intracellular NAD Levels: 40.4 μmol/L - Scroll down to see test report
Cardiovascular Health
- Resting Heart Rate: 52 bpm
- Blood Oxygen Saturation: 99%
Iron Studies
- Serum Iron Level: 21.6umol/L
- Serum Transferrin: 3.06 g/L
- Transferrin Saturation Index: 28%
Vitamin B12
- Serum Vitamin B12: 364 pg/mL
Full Blood Count
- Haemoglobin Estimation: 160 g/L
- Total White Cell Count: 4.1 10*9/L
- Platelet Count: 194 10*9/L
- Neutrophil Count: 1.9 10*9/L
- Lymphocyte Count: 1.6 10*9/L
- Monocyte Count: 0.5 10*9/L
- Eosinophil Count: 0.1 10*9/L
- Basophil: 0.0 10*9/L
- Red Blood Cell Count: 5.17 10*12/L
- Haematocrit: 0.449 L/L
- Mean Corpuscular Volume: 86.8 fl
- Mean Corpusc. Hb. Conc: 356 g/L
- Nucleated Red Blood Cell Count: 0.0 10*9/L
Overview:
The results show a well-balanced and healthy profile. Iron levels indicate efficient oxygen transport throughout the body, supporting muscle and organ function. Vitamin B12 levels are within a healthy range, contributing to energy production and proper nervous system function.
Blood markers reflect effective oxygen delivery and immune system support, with no significant abnormalities detected. Overall, these results suggest that key systems in the body are functioning optimally.
Microbiome
Gut Health Score with Biomesight: 77.39
- Diversity: 93 (satisfactory)
- Probiotics: 74 (needs improvement)
- Commensals: 56 (needs improvement)
- Pathobionts: 85 (satisfactory)
Based on the latest analysis, my gut is well-balanced, with a strong presence of beneficial bacteria like Faecalibacterium prausnitzii, Roseburia, and Akkermansia muciniphila, which support gut lining, reduce inflammation, and aid digestion. However, Bifidobacterium levels are lower than optimal, and some opportunistic bacteria like Bilophila and Sutterella are present, which could contribute to gut imbalances over time.
Microbiome Optimisation Plan
Increase Beneficial Bacteria
- Add more prebiotic-rich foods like onions, garlic, bananas, and oats.
- Include fermented foods (yogurt, kefir, kimchi) to boost Bifidobacterium levels.
Support Gut Lining & Reduce Inflammation
- Consume more polyphenols (green tea, dark chocolate, berries) to support Akkermansia muciniphila.
- Ensure a diet rich in omega-3s to combat inflammation.
Other Tests Completed:
Proteomics , Vitamin E turnover, Cellular Energy Production, ATP-Production, Oral Base Regulation, Oral Acid Regulation, Enamel Protection, Carnosine turnover, Cholesterol Metabolism, Tissue Collagen Integrity, Inflammatory Responce, Renal Efficience, Nitrogen Balance Sugar Regulation, Vitamin A Turnover, Sirtuin Expression, Immune Activation, Vitamin D Turnover, Zinc Turnover
Testing Services:
- Microbiome with Biomesight
- Intracellular NAD and Age Testing with Moleqlar

Jonny's Intracellular NAD levels
- Result: 40.4 μmol/L
- Comparison: This is significantly above the average range for men (12.5 – 29.0 μmol/L).
- Interpretation: Jonny’s NAD+ levels are in the upper percentile of what’s typically seen in young adults.
Downloads:
Supplement Stack
Daily Supplements:
- Vitamin B Complex
- Vitamin C
- Vitamin D3
- Vitamin K2 MK-7
- Omega 3 Algae Oil
- Ashwaganda
- Element 5 NMN
- Resveratrol - Taken with 25ml of EVOO (Extra Virgin Olive Oil)
- Creatine
- Collagen Complex
- Turmeric
- L-Taurine
- Saffron
Morning Drink:
- Electrolytes
- Ceylon Cinnamon
- Chlorella
- Coca Flavanols
- Amino Acid Complex
- Collagen Hydrolysate
Evening Sleep Stack
- Magnesium L-Threonate
- L-Theanene
- Apigenin
- 5HTP
Diet
- Lacto-ovo Vegetarian
- Dinners mostly provided by Grubby Vegan Meal Service (5 per week)
- Eat out occasionally
Calorie Target
- 2,000 - 2,500
Eating Schedule
- Intermittent Fasting
- 1 meal per day
- 3-6PM Eating Window
- 21+ Hours Fasted
Best Practices
- If having breakfast must be high protien, or mostly protien.
- Before eating drink 1tbsp vinegar diluted in water
- Eat veggies first or have a veg starter (Can be anything like Kale, Carrots or even Almonds) Must have fiber.
- Sugar/desserts only after eating
- Eat whole foods where possible (no smoothies)
- No fruit juice or sugary drinks
Drinking
- Water (All day)
- Black Coffee (Morning)
- Peppermint Tea (Daytime)
- Camomile Tea (Evening)
Sleep
Bedtime Routine
- No phone in bedroom
- Complete Daily mindfulness journal
- Read for 15-30 minutes
- Weighted Blanket
- Red or Orange Hue lights
- Earplugs & blackout eye mask
- Air Purifier
Sleep:
- Average Time Asleep : 7 Hours 36 Minutes
- Average REM: 1 Hour 28 Minutes
- Average Core: 5 Hours 27 Minutes
- Average Deep: 41 Minutes
Challenges:
- Caring for a 1-year-old child with night wake-ups has disrupted sleep since September 2023.
- Sleep tracking using an Apple Watch reveals inconsistencies, as it often records periods of being asleep but in reality Jonny is awake with the baby.
Current Tools:
- Using the Apple Watch for sleep tracking to maintain consistency.
Next Steps Under Consideration:
- Exploring alternative, more accurate sleep-tracking devices such as:
- Whoop: Known for advanced recovery and sleep metrics.
- Oura Ring: Offers detailed sleep stage tracking and readiness scores.
- Eight Sleep: A smart mattress system that monitors sleep and optimises temperature for better rest.
The goal is to identify a tool that provides accurate data to improve sleep consistency and quality amidst the challenges of parenting.
Activity
- Dog walks 2 times per day ~ 1 hour total
- Weights: 2-3 times per week
- 100 Press-ups per day 4 days per week
- Run when possible
Work
- Standing Desk
- Grounding Desk Mat
- Air Purifier
- Blue Light Blocking Glasses
Cold & Heat Therapy
- Weekly cold plunge and sauna
Mindfulness
- Meditation where possible
- Affirmations (While Brushing Teeth)
- Night-time journal everyday
Oral Hygiene
- Twice Daily
- Floss
- Interdental Brush
- Tongue Scraper
- Sonic Toothbrush
- Do not rinse
- Hygienist twice yearly
Skincare
- Rotated use of Niacinamide / Vitamin C / Nothing
- Hyaluronic acid daily
- Moisturiser morning and night
- Retinol weekly (Must use SPF next day even if cloudy)
- Cleanse with CeraVe Foaming Cleanser or Hydrating Cleanser Daily
Haircare
- Shampoo & Condition once to twice per week
- Castor Oil once per week
- Micro needle occasionally
- Soon to be using Red light therapy hat
Disclaimer
Jonny Bradley is not a medical professional. The E5 Protocol is shared to provide insight into his personal health journey, with the aim of helping you make informed decisions about your own well-being. Always consult your doctor before making changes to your health routine, and don’t blindly trust everything you see online.
The E5 Protocol is ever-evolving and tailored specifically to Jonny’s needs. For example, Jonny does not currently take iron supplements, despite being a lacto-ovo vegetarian who doesn’t eat meat. He closely monitors his iron levels through regular blood tests and adjusts as necessary.
What works for Jonny may not work for everyone. Always personalise your approach to health based on professional advice and your individual circumstances.
Affiliate Disclaimer: Some links on this website may be affiliate links, meaning we may earn a commission if you make a purchase through them. This comes at no extra cost to you and helps support our work in providing quality content. We only recommend products we believe in, and all opinions remain our own.
Let customers speak for us
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Element 5™ NMN - Nicotinamide Mononucleotide
60 x 500mg Capsules Per Bottle







